Capacity for NADPH regeneration in the leaves of two poplar genotypes differing in ozone sensitivity.
Identifieur interne : 002773 ( Main/Exploration ); précédent : 002772; suivant : 002774Capacity for NADPH regeneration in the leaves of two poplar genotypes differing in ozone sensitivity.
Auteurs : Ata Allah Dghim [France] ; Jennifer Dumont ; Marie-Paule Hasenfratz-Sauder ; Pierre Dizengremel ; Didier Le Thiec ; Yves JolivetSource :
- Physiologia plantarum [ 1399-3054 ] ; 2013.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Populus.
- génétique : Populus.
- métabolisme : Chlorophylle, Dioxyde de carbone, NAD, NADP, Ozone, Phosphoenolpyruvate carboxylase, Ribulose bisphosphate carboxylase.
- Génotype.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Carbon Dioxide, Chlorophyll, NAD, NADP, Ozone, Phosphoenolpyruvate Carboxylase, Ribulose-Bisphosphate Carboxylase.
- enzymology : Populus.
- genetics : Populus.
- Genotype.
Abstract
Cell capacity for cytosolic NADPH regeneration by NADP-dehydrogenases was investigated in the leaves of two hybrid poplar (Populus deltoides × Populus nigra) genotypes in response to ozone (O3 ) treatment (120 ppb for 17 days). Two genotypes with differential O3 sensitivity were selected, based on visual symptoms and fallen leaves: Robusta (sensitive) and Carpaccio (tolerant). The estimated O3 flux (POD0 ), that entered the leaves, was similar for the two genotypes throughout the treatment. In response to that foliar O3 flux, CO2 assimilation was inhibited to the same extent for the two genotypes, which could be explained by a decrease in Rubisco (EC 4.1.1.39) activity. Conversely, an increase in PEPC (EC 4.1.1.31) activity was observed, together with the activation of certain cytosolic NADP-dehydrogenases above their constitutive level, i.e. NADP-G6PDH (EC 1.1.1.49), NADP-ME (malic enzyme) (EC 1.1.1.40) and NADP-ICDH (NADP-isocitrate dehydrogenase) (EC1.1.1.42). However, the activity of non-phosphorylating NADP-GAPDH (EC 1.2.1.9) remained unchanged. From the 11th fumigation day, NADP-G6PDH and NADP-ME profiles made it possible to differentiate between the two genotypes, with a higher activity in Carpaccio than in Robusta. At the same time, Carpaccio was able to maintain high levels of NADPH in the cells, while NADPH levels decreased in Robusta O3 -treated leaves. All these results support the hypothesis that the capacity for cells to regenerate the reducing power, especially the cytosolic NADPH pool, contributes to improve tolerance to high ozone exposure.
DOI: 10.1111/j.1399-3054.2012.01686.x
PubMed: 22978704
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Capacity for NADPH regeneration in the leaves of two poplar genotypes differing in ozone sensitivity.</title><author><name sortKey="Dghim, Ata Allah" sort="Dghim, Ata Allah" uniqKey="Dghim A" first="Ata Allah" last="Dghim">Ata Allah Dghim</name><affiliation wicri:level="4"><nlm:affiliation>UMR1137 EEF, Université de Lorraine, F-54500, Vandoeuvre-lès-Nancy, Cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR1137 EEF, Université de Lorraine, F-54500, Vandoeuvre-lès-Nancy, Cedex</wicri:regionArea><wicri:noRegion>Cedex</wicri:noRegion><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author><author><name sortKey="Dumont, Jennifer" sort="Dumont, Jennifer" uniqKey="Dumont J" first="Jennifer" last="Dumont">Jennifer Dumont</name></author><author><name sortKey="Hasenfratz Sauder, Marie Paule" sort="Hasenfratz Sauder, Marie Paule" uniqKey="Hasenfratz Sauder M" first="Marie-Paule" last="Hasenfratz-Sauder">Marie-Paule Hasenfratz-Sauder</name></author><author><name sortKey="Dizengremel, Pierre" sort="Dizengremel, Pierre" uniqKey="Dizengremel P" first="Pierre" last="Dizengremel">Pierre Dizengremel</name></author><author><name sortKey="Le Thiec, Didier" sort="Le Thiec, Didier" uniqKey="Le Thiec D" first="Didier" last="Le Thiec">Didier Le Thiec</name></author><author><name sortKey="Jolivet, Yves" sort="Jolivet, Yves" uniqKey="Jolivet Y" first="Yves" last="Jolivet">Yves Jolivet</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:22978704</idno><idno type="pmid">22978704</idno><idno type="doi">10.1111/j.1399-3054.2012.01686.x</idno><idno type="wicri:Area/Main/Corpus">002883</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002883</idno><idno type="wicri:Area/Main/Curation">002883</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002883</idno><idno type="wicri:Area/Main/Exploration">002883</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Capacity for NADPH regeneration in the leaves of two poplar genotypes differing in ozone sensitivity.</title><author><name sortKey="Dghim, Ata Allah" sort="Dghim, Ata Allah" uniqKey="Dghim A" first="Ata Allah" last="Dghim">Ata Allah Dghim</name><affiliation wicri:level="4"><nlm:affiliation>UMR1137 EEF, Université de Lorraine, F-54500, Vandoeuvre-lès-Nancy, Cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>UMR1137 EEF, Université de Lorraine, F-54500, Vandoeuvre-lès-Nancy, Cedex</wicri:regionArea><wicri:noRegion>Cedex</wicri:noRegion><orgName type="university">Université de Lorraine</orgName><placeName><settlement type="city">Nancy</settlement><settlement type="city">Metz</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName></affiliation></author><author><name sortKey="Dumont, Jennifer" sort="Dumont, Jennifer" uniqKey="Dumont J" first="Jennifer" last="Dumont">Jennifer Dumont</name></author><author><name sortKey="Hasenfratz Sauder, Marie Paule" sort="Hasenfratz Sauder, Marie Paule" uniqKey="Hasenfratz Sauder M" first="Marie-Paule" last="Hasenfratz-Sauder">Marie-Paule Hasenfratz-Sauder</name></author><author><name sortKey="Dizengremel, Pierre" sort="Dizengremel, Pierre" uniqKey="Dizengremel P" first="Pierre" last="Dizengremel">Pierre Dizengremel</name></author><author><name sortKey="Le Thiec, Didier" sort="Le Thiec, Didier" uniqKey="Le Thiec D" first="Didier" last="Le Thiec">Didier Le Thiec</name></author><author><name sortKey="Jolivet, Yves" sort="Jolivet, Yves" uniqKey="Jolivet Y" first="Yves" last="Jolivet">Yves Jolivet</name></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Carbon Dioxide (metabolism)</term><term>Chlorophyll (metabolism)</term><term>Genotype (MeSH)</term><term>NAD (metabolism)</term><term>NADP (metabolism)</term><term>Ozone (metabolism)</term><term>Phosphoenolpyruvate Carboxylase (metabolism)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Ribulose-Bisphosphate Carboxylase (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Chlorophylle (métabolisme)</term><term>Dioxyde de carbone (métabolisme)</term><term>Génotype (MeSH)</term><term>NAD (métabolisme)</term><term>NADP (métabolisme)</term><term>Ozone (métabolisme)</term><term>Phosphoenolpyruvate carboxylase (métabolisme)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Ribulose bisphosphate carboxylase (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon Dioxide</term><term>Chlorophyll</term><term>NAD</term><term>NADP</term><term>Ozone</term><term>Phosphoenolpyruvate Carboxylase</term><term>Ribulose-Bisphosphate Carboxylase</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chlorophylle</term><term>Dioxyde de carbone</term><term>NAD</term><term>NADP</term><term>Ozone</term><term>Phosphoenolpyruvate carboxylase</term><term>Ribulose bisphosphate carboxylase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genotype</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Génotype</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cell capacity for cytosolic NADPH regeneration by NADP-dehydrogenases was investigated in the leaves of two hybrid poplar (Populus deltoides × Populus nigra) genotypes in response to ozone (O3 ) treatment (120 ppb for 17 days). Two genotypes with differential O3 sensitivity were selected, based on visual symptoms and fallen leaves: Robusta (sensitive) and Carpaccio (tolerant). The estimated O3 flux (POD0 ), that entered the leaves, was similar for the two genotypes throughout the treatment. In response to that foliar O3 flux, CO2 assimilation was inhibited to the same extent for the two genotypes, which could be explained by a decrease in Rubisco (EC 4.1.1.39) activity. Conversely, an increase in PEPC (EC 4.1.1.31) activity was observed, together with the activation of certain cytosolic NADP-dehydrogenases above their constitutive level, i.e. NADP-G6PDH (EC 1.1.1.49), NADP-ME (malic enzyme) (EC 1.1.1.40) and NADP-ICDH (NADP-isocitrate dehydrogenase) (EC1.1.1.42). However, the activity of non-phosphorylating NADP-GAPDH (EC 1.2.1.9) remained unchanged. From the 11th fumigation day, NADP-G6PDH and NADP-ME profiles made it possible to differentiate between the two genotypes, with a higher activity in Carpaccio than in Robusta. At the same time, Carpaccio was able to maintain high levels of NADPH in the cells, while NADPH levels decreased in Robusta O3 -treated leaves. All these results support the hypothesis that the capacity for cells to regenerate the reducing power, especially the cytosolic NADPH pool, contributes to improve tolerance to high ozone exposure.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22978704</PMID><DateCompleted><Year>2014</Year><Month>01</Month><Day>13</Day></DateCompleted><DateRevised><Year>2013</Year><Month>04</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-3054</ISSN><JournalIssue CitedMedium="Internet"><Volume>148</Volume><Issue>1</Issue><PubDate><Year>2013</Year><Month>May</Month></PubDate></JournalIssue><Title>Physiologia plantarum</Title><ISOAbbreviation>Physiol Plant</ISOAbbreviation></Journal><ArticleTitle>Capacity for NADPH regeneration in the leaves of two poplar genotypes differing in ozone sensitivity.</ArticleTitle><Pagination><MedlinePgn>36-50</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1399-3054.2012.01686.x</ELocationID><Abstract><AbstractText>Cell capacity for cytosolic NADPH regeneration by NADP-dehydrogenases was investigated in the leaves of two hybrid poplar (Populus deltoides × Populus nigra) genotypes in response to ozone (O3 ) treatment (120 ppb for 17 days). Two genotypes with differential O3 sensitivity were selected, based on visual symptoms and fallen leaves: Robusta (sensitive) and Carpaccio (tolerant). The estimated O3 flux (POD0 ), that entered the leaves, was similar for the two genotypes throughout the treatment. In response to that foliar O3 flux, CO2 assimilation was inhibited to the same extent for the two genotypes, which could be explained by a decrease in Rubisco (EC 4.1.1.39) activity. Conversely, an increase in PEPC (EC 4.1.1.31) activity was observed, together with the activation of certain cytosolic NADP-dehydrogenases above their constitutive level, i.e. NADP-G6PDH (EC 1.1.1.49), NADP-ME (malic enzyme) (EC 1.1.1.40) and NADP-ICDH (NADP-isocitrate dehydrogenase) (EC1.1.1.42). However, the activity of non-phosphorylating NADP-GAPDH (EC 1.2.1.9) remained unchanged. From the 11th fumigation day, NADP-G6PDH and NADP-ME profiles made it possible to differentiate between the two genotypes, with a higher activity in Carpaccio than in Robusta. At the same time, Carpaccio was able to maintain high levels of NADPH in the cells, while NADPH levels decreased in Robusta O3 -treated leaves. All these results support the hypothesis that the capacity for cells to regenerate the reducing power, especially the cytosolic NADPH pool, contributes to improve tolerance to high ozone exposure.</AbstractText><CopyrightInformation>Copyright © Physiologia Plantarum 2012.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dghim</LastName><ForeName>Ata Allah</ForeName><Initials>AA</Initials><AffiliationInfo><Affiliation>UMR1137 EEF, Université de Lorraine, F-54500, Vandoeuvre-lès-Nancy, Cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dumont</LastName><ForeName>Jennifer</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Hasenfratz-Sauder</LastName><ForeName>Marie-Paule</ForeName><Initials>MP</Initials></Author><Author ValidYN="Y"><LastName>Dizengremel</LastName><ForeName>Pierre</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Le Thiec</LastName><ForeName>Didier</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Jolivet</LastName><ForeName>Yves</ForeName><Initials>Y</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>09</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Physiol Plant</MedlineTA><NlmUniqueID>1256322</NlmUniqueID><ISSNLinking>0031-9317</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0U46U6E8UK</RegistryNumber><NameOfSubstance UI="D009243">NAD</NameOfSubstance></Chemical><Chemical><RegistryNumber>1406-65-1</RegistryNumber><NameOfSubstance UI="D002734">Chlorophyll</NameOfSubstance></Chemical><Chemical><RegistryNumber>142M471B3J</RegistryNumber><NameOfSubstance UI="D002245">Carbon Dioxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>53-59-8</RegistryNumber><NameOfSubstance UI="D009249">NADP</NameOfSubstance></Chemical><Chemical><RegistryNumber>66H7ZZK23N</RegistryNumber><NameOfSubstance UI="D010126">Ozone</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.1.1.31</RegistryNumber><NameOfSubstance UI="D010730">Phosphoenolpyruvate Carboxylase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.1.1.39</RegistryNumber><NameOfSubstance UI="D012273">Ribulose-Bisphosphate Carboxylase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002245" MajorTopicYN="N">Carbon Dioxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002734" MajorTopicYN="N">Chlorophyll</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005838" MajorTopicYN="N">Genotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009243" MajorTopicYN="N">NAD</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009249" MajorTopicYN="N">NADP</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010126" MajorTopicYN="N">Ozone</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010730" MajorTopicYN="N">Phosphoenolpyruvate Carboxylase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012273" MajorTopicYN="N">Ribulose-Bisphosphate Carboxylase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>06</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>06</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>9</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>9</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>1</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22978704</ArticleId><ArticleId IdType="doi">10.1111/j.1399-3054.2012.01686.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Metz</li><li>Nancy</li></settlement><orgName><li>Université de Lorraine</li></orgName></list><tree><noCountry><name sortKey="Dizengremel, Pierre" sort="Dizengremel, Pierre" uniqKey="Dizengremel P" first="Pierre" last="Dizengremel">Pierre Dizengremel</name><name sortKey="Dumont, Jennifer" sort="Dumont, Jennifer" uniqKey="Dumont J" first="Jennifer" last="Dumont">Jennifer Dumont</name><name sortKey="Hasenfratz Sauder, Marie Paule" sort="Hasenfratz Sauder, Marie Paule" uniqKey="Hasenfratz Sauder M" first="Marie-Paule" last="Hasenfratz-Sauder">Marie-Paule Hasenfratz-Sauder</name><name sortKey="Jolivet, Yves" sort="Jolivet, Yves" uniqKey="Jolivet Y" first="Yves" last="Jolivet">Yves Jolivet</name><name sortKey="Le Thiec, Didier" sort="Le Thiec, Didier" uniqKey="Le Thiec D" first="Didier" last="Le Thiec">Didier Le Thiec</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Dghim, Ata Allah" sort="Dghim, Ata Allah" uniqKey="Dghim A" first="Ata Allah" last="Dghim">Ata Allah Dghim</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002773 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002773 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:22978704
|texte= Capacity for NADPH regeneration in the leaves of two poplar genotypes differing in ozone sensitivity.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:22978704" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |